CovidV1, Main, Exploration, bibRecord, 001181

Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells

Identifieur interne : 001181 ( Main/Exploration ); précédent : 001180; suivant : 001182

Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells

Auteurs : Takayuki Murata [Japon] ; Yasuhiro Takashima [Japon] ; Xuen Xuan [Japon] ; Haruki Otsuka [Japon]

Source :

Virus Research [ 0168-1702 ] ; 1999.

RBID : ISTEX:6045C6463913FA654B074DB6EB111853034794EA

English descriptors

KwdEn :
- Adsorption, Bovine herpesvirus-1, Immediate early protein, Penetration, Quantitative competitive PCR.
Teeft :
- Adsorption, Agarose, Akabane virus, Attachment period, Bicp0, Bicp0 genes, Bicp4, Bicp4 antigen, Bluetongue virus, Bovine, Bovine herpesvirus, Cell lines, Cell surface, Competitor plasmid, Equal amounts, Glycoprotein, Growth behavior, Heparan, Heparan sulfate, Heparin, Heparin wash, Herpes, Herpes simplex virus, Herpes simplex virus type, Herpesvirus, Infectious centers, Mdbk, Mdbk cells, Monolayers, Murata, Mutant, Nacl, Nuclear transport, Other hand, Permissive mdbk, Permissive mdbk cells, Plasmid, Pseudorabies virus, Qcpcr, Recombinant, Replication, Simplex, Small fraction, Sulfate, Trypsin, Trypsin treatment, Various times, Viral, Virion, Virol, Virology, Virus, Virus entry, Virus research, Wild type.

Abstract

Abstract: Bovine herpesvirus-1 (BHV-1) can replicate well in bovine-derived cell lines such as Madin Darby bovine kidney (MDBK) but grows poorly in hamster lung (HmLu-1). Virus replication, DNA synthesis, and immediate-early gene expression are severely restricted in HmLu-1. We compared adsorption and penetration of BHV-1 in permissive MDBK and semi-permissive HmLu-1 cells. At a low multiplicity of infection, BHV-1 attached to permissive MDBK cells twice as much as to HmLu-1. The presence of heparin inhibited the attachment of BHV-1 to MDBK cells by about 60%, but over 90% of the attachment was inhibited in HmLu-1. To investigate the penetration of BHV-1, we performed the quantitative measurement of viral DNA by quantitative competitive (QC)PCR in infected cells. In MDBK cells, virions attached to the cell surface, penetrated into the cells and were transported to the nucleus. However in HmLu-1, only a small fraction of the virions attached to the cell surface were allowed to penetrate. Our results indicated that the replication of BHV-1 in semi-permissive HmLu-1 was not dramatically restricted at one certain point but at some various stages including adsorption and penetration.

Url:

https://api.istex.fr/ark:/67375/6H6-RZ3R4CSS-K/fulltext.pdf

DOI: 10.1016/S0168-1702(99)00023-4

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000827
to stream Istex, to step Curation: 000807
to stream Istex, to step Checkpoint: 000374
to stream Main, to step Merge: 001194
to stream Main, to step Curation: 001181

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells</title>
<author><name sortKey="Murata, Takayuki" sort="Murata, Takayuki" uniqKey="Murata T" first="Takayuki" last="Murata">Takayuki Murata</name>
</author>
<author><name sortKey="Takashima, Yasuhiro" sort="Takashima, Yasuhiro" uniqKey="Takashima Y" first="Yasuhiro" last="Takashima">Yasuhiro Takashima</name>
</author>
<author><name sortKey="Xuan, Xuen" sort="Xuan, Xuen" uniqKey="Xuan X" first="Xuen" last="Xuan">Xuen Xuan</name>
</author>
<author><name sortKey="Otsuka, Haruki" sort="Otsuka, Haruki" uniqKey="Otsuka H" first="Haruki" last="Otsuka">Haruki Otsuka</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:6045C6463913FA654B074DB6EB111853034794EA</idno>
<date when="1999" year="1999">1999</date>
<idno type="doi">10.1016/S0168-1702(99)00023-4</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-RZ3R4CSS-K/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000827</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000827</idno>
<idno type="wicri:Area/Istex/Curation">000807</idno>
<idno type="wicri:Area/Istex/Checkpoint">000374</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000374</idno>
<idno type="wicri:doubleKey">0168-1702:1999:Murata T:growth:behavior:of</idno>
<idno type="wicri:Area/Main/Merge">001194</idno>
<idno type="wicri:Area/Main/Curation">001181</idno>
<idno type="wicri:Area/Main/Exploration">001181</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells</title>
<author><name sortKey="Murata, Takayuki" sort="Murata, Takayuki" uniqKey="Murata T" first="Takayuki" last="Murata">Takayuki Murata</name>
<affiliation wicri:level="4"><country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
<orgName type="university">Université de Tokyo</orgName>
<placeName><settlement type="city">Tokyo</settlement>
<region type="province">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Takashima, Yasuhiro" sort="Takashima, Yasuhiro" uniqKey="Takashima Y" first="Yasuhiro" last="Takashima">Yasuhiro Takashima</name>
<affiliation wicri:level="4"><country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
<orgName type="university">Université de Tokyo</orgName>
<placeName><settlement type="city">Tokyo</settlement>
<region type="province">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Xuan, Xuen" sort="Xuan, Xuen" uniqKey="Xuan X" first="Xuen" last="Xuan">Xuen Xuan</name>
<affiliation wicri:level="1"><country xml:lang="fr">Japon</country>
<wicri:regionArea>The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555</wicri:regionArea>
<wicri:noRegion>Hokkaido 080-8555</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Otsuka, Haruki" sort="Otsuka, Haruki" uniqKey="Otsuka H" first="Haruki" last="Otsuka">Haruki Otsuka</name>
<affiliation wicri:level="1"><country wicri:rule="url">Japon</country>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
<orgName type="university">Université de Tokyo</orgName>
<placeName><settlement type="city">Tokyo</settlement>
<region type="province">Région de Kantō</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virus Research</title>
<title level="j" type="abbrev">VIRUS</title>
<idno type="ISSN">0168-1702</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1999">1999</date>
<biblScope unit="volume">61</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="29">29</biblScope>
<biblScope unit="page" to="41">41</biblScope>
</imprint>
<idno type="ISSN">0168-1702</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0168-1702</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adsorption</term>
<term>Bovine herpesvirus-1</term>
<term>Immediate early protein</term>
<term>Penetration</term>
<term>Quantitative competitive PCR</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Adsorption</term>
<term>Agarose</term>
<term>Akabane virus</term>
<term>Attachment period</term>
<term>Bicp0</term>
<term>Bicp0 genes</term>
<term>Bicp4</term>
<term>Bicp4 antigen</term>
<term>Bluetongue virus</term>
<term>Bovine</term>
<term>Bovine herpesvirus</term>
<term>Cell lines</term>
<term>Cell surface</term>
<term>Competitor plasmid</term>
<term>Equal amounts</term>
<term>Glycoprotein</term>
<term>Growth behavior</term>
<term>Heparan</term>
<term>Heparan sulfate</term>
<term>Heparin</term>
<term>Heparin wash</term>
<term>Herpes</term>
<term>Herpes simplex virus</term>
<term>Herpes simplex virus type</term>
<term>Herpesvirus</term>
<term>Infectious centers</term>
<term>Mdbk</term>
<term>Mdbk cells</term>
<term>Monolayers</term>
<term>Murata</term>
<term>Mutant</term>
<term>Nacl</term>
<term>Nuclear transport</term>
<term>Other hand</term>
<term>Permissive mdbk</term>
<term>Permissive mdbk cells</term>
<term>Plasmid</term>
<term>Pseudorabies virus</term>
<term>Qcpcr</term>
<term>Recombinant</term>
<term>Replication</term>
<term>Simplex</term>
<term>Small fraction</term>
<term>Sulfate</term>
<term>Trypsin</term>
<term>Trypsin treatment</term>
<term>Various times</term>
<term>Viral</term>
<term>Virion</term>
<term>Virol</term>
<term>Virology</term>
<term>Virus</term>
<term>Virus entry</term>
<term>Virus research</term>
<term>Wild type</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Bovine herpesvirus-1 (BHV-1) can replicate well in bovine-derived cell lines such as Madin Darby bovine kidney (MDBK) but grows poorly in hamster lung (HmLu-1). Virus replication, DNA synthesis, and immediate-early gene expression are severely restricted in HmLu-1. We compared adsorption and penetration of BHV-1 in permissive MDBK and semi-permissive HmLu-1 cells. At a low multiplicity of infection, BHV-1 attached to permissive MDBK cells twice as much as to HmLu-1. The presence of heparin inhibited the attachment of BHV-1 to MDBK cells by about 60%, but over 90% of the attachment was inhibited in HmLu-1. To investigate the penetration of BHV-1, we performed the quantitative measurement of viral DNA by quantitative competitive (QC)PCR in infected cells. In MDBK cells, virions attached to the cell surface, penetrated into the cells and were transported to the nucleus. However in HmLu-1, only a small fraction of the virions attached to the cell surface were allowed to penetrate. Our results indicated that the replication of BHV-1 in semi-permissive HmLu-1 was not dramatically restricted at one certain point but at some various stages including adsorption and penetration.</div>
</front>
</TEI>
<affiliations><list><country><li>Japon</li>
</country>
<region><li>Région de Kantō</li>
</region>
<settlement><li>Tokyo</li>
</settlement>
<orgName><li>Université de Tokyo</li>
</orgName>
</list>
<tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Murata, Takayuki" sort="Murata, Takayuki" uniqKey="Murata T" first="Takayuki" last="Murata">Takayuki Murata</name>
</region>
<name sortKey="Otsuka, Haruki" sort="Otsuka, Haruki" uniqKey="Otsuka H" first="Haruki" last="Otsuka">Haruki Otsuka</name>
<name sortKey="Otsuka, Haruki" sort="Otsuka, Haruki" uniqKey="Otsuka H" first="Haruki" last="Otsuka">Haruki Otsuka</name>
<name sortKey="Takashima, Yasuhiro" sort="Takashima, Yasuhiro" uniqKey="Takashima Y" first="Yasuhiro" last="Takashima">Yasuhiro Takashima</name>
<name sortKey="Xuan, Xuen" sort="Xuan, Xuen" uniqKey="Xuan X" first="Xuen" last="Xuan">Xuen Xuan</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001181 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001181 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    CovidV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:6045C6463913FA654B074DB6EB111853034794EA
   |texte=   Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells
}}

This area was generated with Dilib version V0.6.33.
Data generation: Fri Mar 27 18:14:15 2020. Site generation: Sun Jan 31 15:15:08 2021

	Serveur d'exploration Covid
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration Covid

Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells

Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri